31/08/2025 - Cytokinetics Incorporated: Effect of Aficamten Compared with Metoprolol on Cardiac Structure and Function in Symptomatic Obstructive Hypertrophic Cardiomyopathy: A Prespecified Analysis of MAPLE-HCM

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Effect of Aficamten Compared with Metoprolol on Cardiac Structure and Function in Symptomatic Obstructive Hypertrophic Cardiomyopathy:

A prespecified analysis of MAPLE-HCM



Sheila M. Hegde, MD MPH1,2, Xiaowen Wang, MD, MPH, Pablo Garcia-Pavia, MD, PhD, Stoyan Getchevski, MD, Ahmad Masri, MD, Bela Merkely, MD, Michael E. Nassif, MD, Maria Luisa Peña-Peña, MD, Roberto Barriales-Villa, MD, PhD, Ozlem Bilen, MD,

Melissa Burroughs, MD, Brian Claggett, PhD, Juan Pablo Costabel, MD, Edileide de Barros Correia, MD, Anne M. Dybro, MD, PhD, Perry Elliott, MBBS, MD, Neal K. Lakdawala, MD, Amy Mann, BA, Martin S. Maron, MD, Ajith Nair, MD, Steen H. Poulsen, MD, Patricia Reant, MD, PhD, P. Christian Schulze, MD; Andrew Wang, MD, Regina Sohn, MD, PhD, Indrias Berhane, PhD,

Stephen B. Heitner, MD, Daniel L. Jacoby, MD, Stuart Kupfer, MD, Fady I. Malik, MD, PhD, Amy Wohltman, ME, Michael A. Fifer, MD, Scott D. Solomon, MD, on behalf of the MAPLE-HCM Investigators

1Cardiovascular Division, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.

2University of Texas Southwestern Medical Center, Dallas, TX, USA.

AUGUST 31, 2025



Background

  • Beta-blockers have served as the first-line therapy for symptomatic obstructive HCM (oHCM) for decades

  • Aficamten is an investigational, next-in-class CMI, a small-molecule selective inhibitor of the cardiac myosin ATPase, that targets and reduces hypercontractility

  • MAPLE-HCM is a phase 3 head-to-head comparative efficacy and safety study of monotherapy with aficamten vs metoprolol in adults with symptomatic oHCM



    Baseline Characteristics

  • 175 participants

  • Mean age: 58 ± 13 years

  • 42% Female

  • 80% White, 14% Asian

  • 70% prior B-blocker use

  • 13% CCB use

Primary Endpoint (peak VO )

Aficamten 5‒20 mg + placebo for metoprolol (n=88)

Metroprolol 50‒200 mg + placebo

for aficamten (n=87)

Adults (aged 18‒85 years) with:

  • Diagnosis of oHCM

  • NYHA class II or III

  • Resting LVOT-G ≥30 mmHg and/or post-Valsalva ≥50 mmHg

  • LVEF ≥60% at screening

  • pVO2 <100%

Randomization 1:1

End of Study

Dose adjustment based on echo and vital signs*

Serial echo Washout

2

Mean Change from Baseline to Week 24



Mean (95% CI) pVO2(mL/kg/min)

22

LSM difference (SE) vs metoprolol

2.3 mL/kg/min (0.39)

P<0.0001

21

20

2-4 weeks (2-week SoC washout)

Study Visits

Screening D1 W2*W4* W6* W8 W12 W16 W20 W24 W28

19

18

17

BL Week 24

*Metoprolol doses were uptitrated in 50 mg increments from 50 to 200 mg. Aficamten doses were uptitrated in 5 mg increments from 5 to 20 mg.

Aficamten

Metoprolol

CCB, calcium channel blocker; CMI, cardiac myosin inhibitor; D, day; echo, echocardiogram; KCCQ, Kansas City Cardiomyopathy Questionnaire; LSM, least squared mean; LVEF, left ventricular ejection fraction; LVOT, left ventricular outflow tract;; NT-proBNP, N-terminal pro-B-type natriuretic peptide; NYHA, New York Heart Association; oHCM, obstructive hypertrophic cardiomyopathy; pVO2, peak oxygen uptake; SoC, standard of care; W, week.



Systolic Function and Gradients

Resting LVOT Gradient



Valsalva LVOT Gradient



Resting LVOT Gradient (mmHg)

Valsalva LVOT Gradient (mmHg)

80 P < 0.001

70

60

50

40

30

20

10

0 Washout

0 2 4 6 8 12 16 20 24 28

Weeks

80 P < 0.001

70

60

50

40

30

20

10

0 Washout

0 2 4 6 8 12 16 20 24 28

Weeks

−30 mmHg (−37, −23)

−35 mmHg (−44, −26)

Resting LVOT-G Valsalva LVOT-G

Metoprolol Aficamten

LV Ejection Fraction (%)

Absolute LV Global Circumferental Strain (%)

Absolute LV Global Longitudinal Strain (%)

LV Ejection Fraction

Absolute LV GCS

Absolute LV GLS

−5% (−5, −3)

Treatment-corrected difference (95% CI) is adjusted for baseline echo measure, treatment, exercise mode (bicycle vs treadmill) and stratification by time of diagnosis (recent [Group 1] vs chronic [Group 2]) with corresponding P values at 24 weeks.

Horizontal dashed lines represent thresholds for gradients and normal values for LVEF.

GCS, global circumferential strain; GLS, global longitudinal strain; LV, left ventricular; LVEF, left ventricular ejection fraction; LVOT-G, left ventricular outflow tract gradient.

−1.8% (−1.8, −0.5)

−2.5% (−3.7, −1.3)

70

P < 0.001

60

55

45

35

34

33

32

31

30

29

28

P < 0.001

P < 0.001

18

65

17

16

50

15

Washout

Washout

14

Washout

0 2 4 6 8

12 16

Weeks

20 24 28

0

12

24 28

0

12

24 28

Weeks

Weeks



LVEF

Absolute LV GCS Absolute LV GLS





Cardiac Structure

22

16

P = 0.04

16

P < 0.001

21

20

19

18

17

16

15

15

14

14

13

13

12

12

Washout

11

Washout

11

Washout

0 2 4 6 8 12 16 20 24 28 0 2 4 6 8 12 16 20 24 28 0 2 4 6 8 12 16 20 24 28

Weeks Weeks Weeks

+1.6 mL/m2 (0.7, 2.5)

−0.8 mm (−1.5 −0.0)

−1.0 mm (−1.8, −0.20)

0.02

P =

Maximum Wall Thickness (mm)

Inferolateral Wall Thickness (mm)

LV End Systolic Volume Index (ml/m2)

Maximal Wall Thickness Inferolateral Wall Thickness LV ESVi

Metoprolol Aficamten

Not Shown: Interventricular Septal Wall LV Mass Index

Maximal Wall Thickness

Inferolateral Wall


LV ESVi

Treatment-corrected difference (95% CI) is adjusted for baseline echo measure, treatment, exercise mode (bicycle vs treadmill) and stratification by time of diagnosis (recent [Group 1] vs chronic [Group 2]) with corresponding P values at 24 weeks.

LV, left ventricular; LV; ESVi, left ventricular end systolic volume index.





Diastolic Function

LAVi Lateral E/e Septal E/e



P < 0.001

P < 0.001

P < 0.001

45

20

20

18

18

40

16

16

35

14

14

12

12

30

Washout

10

Washout

10

Washout

0

12

Weeks

24 28

0

12

Weeks

24 28

0

12

Weeks

24 28

−3.1 (−4.5, −1.7)

−2.8 (−4.0, −1.6)

−7.0 mL/m2 (−9.1, −4.8)

Left Atrial Volume Index (mm2)

LAVi

Lateral E/e'

Septal E/e'

Lateral e Septal e Lateral E/e Septal E/e

Metoprolol Aficamten

Treatment-corrected difference (95% CI) is adjusted for baseline echo measure, treatment, exercise mode (bicycle vs treadmill) and stratification by time of diagnosis (recent [Group 1] vs chronic [Group 2]) with corresponding P values at 24 weeks.

Horizontal dashed lines represent normal values left atrial volume index, lateral E/e′ , and septal E/e′.

LAVi, left atrial volume index; e', mitral annular early diastolic velocity; E/e', ratio between early mitral inflow velocity and septal and lateral mitral annular early diastolic velocity



Disclaimer

Cytokinetics Incorporated published this content on August 31, 2025, and is solely responsible for the information contained herein. Distributed via Public Technologies (PUBT), unedited and unaltered, on August 31, 2025 at 18:06 UTC.

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